Erg protein levels are decreased in HUVECs treated with Erg GeneBlocs.

Nuclear extracts from cells treated with 50 nM Erg GeneBloc (GB566) or control GeneBloc (GBC3.3) for 24, 48, or 72 hours (A) and 50 nM control GeneBloc (GBC3.3), Mismatch GeneBloc (GB478), or Erg GeneBloc (GB566) for 24 hours (B) were generated as described (see “Materials and methods”). Western blot analysis with anti-Erg and anti-Oct monoclonal antibodies was performed, and the intensity of the bands was measured by densitometry. Levels of the transcription factor Oct were used to normalize for differences in loading. The ratio between Erg and Oct in the Erg GeneBloc and in the Control samples is shown below using data from densitometry.