Erg GeneBloc decreases ICAM-2, RhoA, TSP, vWF, and SPARC mRNA levels in HUVECs.

RNA was prepared from HUVECs treated with Erg-specific (GB566) or control (GB478) GeneBloc. Real-time PCR analysis (TaqMan for ICAM-2 and RhoA or Lightcycler for TSP, vWF, and SPARC) was used to quantify mRNA levels. Results for each gene are shown as a ratio to GAPDH mRNA levels, which were identified as the least variable control genes in these cells (see “Results”). 100% represents Erg mRNA level in HUVECs treated with the mismatch control GeneBloc (GB478).