Fig. 5.
Intracellular half-life of His-M195FANCF in FA group F cells.
(A) His-M195FANCF protein (30 μg) was added to EUFA698 lymphoblasts (1 × 106/mL) in complete medium at 4°C and internalized by warming to 37°C, and the intracellular fate of the fusion proteins was assessed by Western analysis with anti-FANCF antibody. Lanes are: a, 2 minutes chase; b, 5 minutes chase; c, 1 hour chase; d, 2 hours chase; e, 4 hours chase; f, 8 hours chase; g, 24 hours chase; and h, no chase. The position of 68.5-kd His-M195FANCF is shown (arrow). (B) The representative gel shown in (A) was scanned by densitometry to derive the graph. Data corresponding to lanes b-f are shown. Remaining time points are omitted for clarity.
