Correction of the MMC-hypersensitive phenotype of FA group F cells by protein transduction.

Parental or stably transfected (with pREP4-CD33 or pREP4-FANCF, as indicated) EUFA698 cells (FA-F), HSC72 cells (FA-A), HSC536 cells (FA-C), and normal lymphoblasts (NLs) were used in these experiments. (A) Trypan blue exclusion was used to assess the viability of these cells in the presence of continuous exposure to MMC. Where indicated, His-M195FANCF (0.1 mg/mL) was added to cultures daily for 6 days. (B) Selective rescue of CD33⁺ FA-F cells but not cells from other FA complementation groups with His-M195FANCF. The IC₅₀ values are derived from standard growth curves. The mean values of each experiment performed in triplicate are shown.