Fig. 2.
Scatchard plot for the binding of 125I-rhEPO to hEPO-specific magnetic beads.
The line represents the correlation between bound and free125I-EPO ratio versus the tracer activity bound to the beads. Kd for rhEPO was 2.7 × 10−11 mol/L, and maximal binding capacity was 2.1 × 10−11 mol/L or approximately 50 fmol/mg beads.