The production of IL-12 p70 by BM-donor–derived DCs isolated from irradiated recipient mouse spleen.

The Ly 5.2⁺ donor BM-derived CD8α⁺ DCs from BM reconstituted irradiated Ly 5.1 recipient mice 2 weeks after BM transfer, or CD8α⁺ DCs from normal mice, were purified from the spleens. The purified CD8α⁺ DCs (10⁵) were cultured with an optimized IL-12 stimulus; the supernatant was collected after overnight culture and assayed for IL-12 p70 content by ELISA. The result is representative of 2 such experiments. Error bars represent the range of duplicate samples.