The production of splenic DC populations by BM myeloid-committed precursors.

Purified Ly 5.2⁺ BM CMPs and GMPs were IV injected into lethally irradiated Ly 5.1 recipients. At various times after precursor cell transfer, DCs were enriched from the recipient spleen, and donor-derived DC populations were analyzed as described in Figure 4. (A) Examples of the phenotype of precursor-derived DCs 2 weeks after IV transfer of CMPs or GMPs are shown. (B) The absolute number of each donor-derived DC population was calculated at each time point, and these are presented in a kinetic manner. The data presented here are the means of 2 to 3 such experiments for each time point, and each experimental group included 3 mice.