BCL11A amplification and overexpression in Hodgkin and non-Hodgkin lymphoma.

(A) BCL11A amplification in a primary case of B-NHL. FISH with 2 pooled BCL11A-specific BACs RP11-440P05 and RP11-158I21 (green signal) and 2 pooled REL-specific YAC clones 747H5 and 927G9 (red signal) on interphase nuclei from a case of relapsed t(14;18)(q32.3;q21.3)–negative nodal follicular lymphoma showing coamplification of BCL11A and REL. Chromosome analysis revealed 2 abnormal chromosomes 2, add(2)(p14∼15) and der(1)t(1;2)(p11;p11)trp(2)(p12p13). (B) Northern blot analysis of malignant lymphoma cell lines. Burkitt lymphoma cell lines Wien 133 and Raji, and HD cell line L540, showed low-level BCL11Aexpression. HD cell lines L428 and KM-H2 showed overexpression ofBCL11A. In L428, this was predominantly ofBCL11A_L, whereas in KM-H2 all 3 isoforms were expressed at similar levels.