Platelet deposition onto vWf–collagen 1 surfaces in the presence of a constant ARMX concentration and increasing concentrations of A3P5P.
Heparinized whole blood was incubated at 37°C for 5 minutes with saline (control, closed squares), 0.5 μM ARMX + either 0.5 μM A3P5P (open diamonds), 1 μM A3P5P (closed circles), 10 μM A3P5P (closed triangles), or 0.5 μM ARMX + 100 μM A3P5P (open squares) before perfusion over a vWf–collagen 1 surface at 3000 s−1 for 1 minute at 37°C. Mepacrine-labeled fluorescent platelets were videotaped in real time using an inverted epi-fluorescence microscope with an attached SIT video camera. Images were digitized to quantify mural thrombus formation. Values reported are mean ± SEM of the total number of platelets in a single view of 37 000 μm2. *Significant difference from control;P < .05.
