Platelet deposition onto vWf–collagen 1 after 60 seconds of perfusion at 3000 s⁻¹.

Heparinized whole blood was incubated at 37°C for 5 minutes with saline (control), or with each compound or combination tested, before perfusion over vWf–collagen 1 surface at 3000 s⁻¹ for 60 seconds at 37°C. Mepacrine-labeled fluorescent platelets were videotaped in real time using an inverted epi-fluorescence microscope with an attached SIT video camera. Images were digitized to quantify mural thrombus formation. Values reported are mean ± SEM of the total number of platelets deposited in a 37 000 μm²area. *Significant difference from control;P < .05.