Fig. 1.
Fig. 1. The. / tetO-HOXA10 transgenic construct and characterization of transgenic mice. (A) The HOXA10 construct containing the human HOXA10 cDNA under the control of the PhCMV*-1, a minimal promoter from hCMV fused to the tetO sequences. The intron-polyA (pA) sequence is derived from the rabbitβ-globin gene. The picture is not drawn to scale. (B) Southern blot analysis showing 3 of the transgenictetO-HOXA10 mouse lines. Approximately 10 μg genomic DNA was digested with BamHI and hybridized with a HOXA10-specific probe. The band in the control lane corresponds to 10 copies (cop) of the construct mixed together with genomic DNA from wild-type control mice.

The

tetO-HOXA10 transgenic construct and characterization of transgenic mice. (A) The HOXA10 construct containing the human HOXA10 cDNA under the control of the PhCMV*-1, a minimal promoter from hCMV fused to the tetO sequences. The intron-polyA (pA) sequence is derived from the rabbitβ-globin gene. The picture is not drawn to scale. (B) Southern blot analysis showing 3 of the transgenictetO-HOXA10 mouse lines. Approximately 10 μg genomic DNA was digested with BamHI and hybridized with a HOXA10-specific probe. The band in the control lane corresponds to 10 copies (cop) of the construct mixed together with genomic DNA from wild-type control mice.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal