Fig. 2.
Fig. 2. The experimental design using the tTA vector. / (A) The NIT-GFP vector used in the experiment for transducingtetO-HOXA10 transgenic BM cells. LTR indicates long terminal repeats; neo, neomycin-resistance gene; IRES, internal ribosomal entry site; tTA, tetracycline transactivator; tetO, PhCMV*-1, minimal promoter fused to the tet operator (tetO) sequences; EGFP, enhanced green fluorescent protein; prestim, prestimulated; doxy, doxycycline; Liq, liquid; rad, cGy. (B) A schematic overview of the experimental design.

The experimental design using the tTA vector.

(A) The NIT-GFP vector used in the experiment for transducingtetO-HOXA10 transgenic BM cells. LTR indicates long terminal repeats; neo, neomycin-resistance gene; IRES, internal ribosomal entry site; tTA, tetracycline transactivator; tetO, PhCMV*-1, minimal promoter fused to the tet operator (tetO) sequences; EGFP, enhanced green fluorescent protein; prestim, prestimulated; doxy, doxycycline; Liq, liquid; rad, cGy. (B) A schematic overview of the experimental design.

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