Induction of transgenic HOXA10 expression in vitro.

Induction of HOXA10 transgene expression in 4 different transgenic mouse lines. tetO-HOXA10 BM cells were transduced with the NIT-GFP vector and then grown in selective medium in the presence (+) or absence (−) of doxycycline (2 μg/mL) for 5 days. RT-PCR was performed on RNA isolated from cultured cells using the A10 and BG primers amplifying a 950-bp band. For RNA/DNA control, HPRT primers were used. PCR was also performed on isolated RNA to verify the absence of genomic DNA contamination (data not shown).