Induced expression of HOXA10 affects colony formation in methylcellulose culture.

(A) Depicted is the frequency of CFU-M, CFU-GM, CFU-Mix, and the unique megakaryocytic-blast colonies. BM cells from tetO-HOXA10 and control mice were transduced with the NIT-GFP vector and plated out on methylcellulose. On day 11 to 12, colonies (n = 434 for A10^−doxy, n = 346 for A10^+doxy, n = 158 for control cells) were scored on the plates. The morphology of a number of the colonies was further verified by picking single G418-resistant colonies for examination by Wright-Giemsa staining. Results are expressed as means ± SD from 4 independent experiments. (B) Day 12 methylcellulose colonies, derived from NIT-GFP–transduced tetO-HOXA10 progenitor cells grown in the absence of doxycycline. (Bi) Colony expressing the GFP marker gene (original magnification × 40). (Bii) Wright-Giemsa staining of cytospin preparation of methylcellulose colony containing megakaryocytes (Meg) and blastlike (b) cells (CFU-Meg/blast) formed by cells overexpressing HOXA10 (original magnification × 200).