Regulation of HOXA10 and GFP expression in vivo.
Directly after NIT-GFP transduction, 106tetO-HOXA10 cells were injected into lethally irradiated recipients. The expression of GFP and HOXA10 was then monitored by FACS and RT-PCR, respectively. Representative results from one recipient mouse receiving transplants of NIT-GFP–transduced BM cells from line 2-5 are presented. (A) Percentage of GFP-expressing cells in the peripheral blood of the recipient 10 weeks after transplantation, in the absence (d0 and d19) or presence (d7) of doxycycline. (B) HOXA10 expression levels in the peripheral blood at the indicated time points. The levels were measured by performing RT-PCR on total RNA isolated from leukocytes. Absence of DNA contamination was also verified (data not shown).
