Fig. 7.
The inducible proliferative effect on CFU-Sd12 progenitors is reversible.
HOXA10 production was induced for 7 days and then either down-regulated (+Doxy) or the induction was kept on (−Doxy). (A) An aliquot of transduced transgenic cells that had been cultured for 7 days in the absence of doxycycline was taken and split into 2, 5-day cultures with or without doxycycline. The cells were then injected into lethally irradiated recipients for a CFU-Sd12 assay. (B) Number of day 12 spleen colonies per 10 000 input cells. Results are shown as number of colonies ± SD from 1 of 2 experiments. Doxy indicates doxycycline; n, number of mice.
