GFP

⁺ CTLs were detected in tissues from engrafted recipients but not in recipients who had rejected donor grafts.Shown are histograms from representative experiments for detection of GFP⁺ and CD3⁺ cells obtained from blood, spleen, lymph node, lung, liver, and heart at necropsy. The boxes circumscribe CD3⁺ cells. The percent of CD3⁺cells that are GFP⁺ is indicated in each histogram. (A) Dog E746 engrafted and developed GVHD following 9.2 Gy TBI and transplantation of allogeneic marrow and GFP⁺ CTLs. GFP⁺ cells were detected in the blood, spleen, lymph node, lung, and liver but not in heart. In this recipient, 6.4% of CD3⁺ cells from the liver were GFP⁺. (B) Dog E815 rejected the donor graft; no GFP⁺ CTLs were detected in any tissue. (C) Dog E753 received autologous marrow and GFP⁺ CTLs following 9.2 Gy TBI. Here, 6.9% of CD3⁺ cells from the liver were GFP⁺.