Fig. 2.
Fig. 2. Detection of CD2 in neoplastic MCs by in situ immunostaining. / (A,B) Immunohistochemical detection of CD2 in BM MCs in a patient with SM. The BM biopsy section was first stained with CD2 mAb AB75 (A), demounted, and then stained with mAb G3 against tryptase (B). As visible, the spindle-shaped tryptase-positive MCs were found to be immunoreactive for CD2. Original magnification approximately × 500. (C,D) Immunocytochemistry of HMC-1 cells. Sorted HMC-1 subsets, ie, CD2+ HMC-1 cells (C) and CD2− HMC-1 cells (D), were spun on cytospin slides and stained with CD2 mAb 6F10.3 as described in the text. Immunocytochemistry confirmed expression of the CD2 protein in the cytoplasm of CD2+ HMC-1 cells and absence of (cytoplasmic) CD2 in the surface CD2− HMC-1 cells. Note granular staining pattern of CD2+ HMC-1 cells. Original magnification approximately × 250.

Detection of CD2 in neoplastic MCs by in situ immunostaining.

(A,B) Immunohistochemical detection of CD2 in BM MCs in a patient with SM. The BM biopsy section was first stained with CD2 mAb AB75 (A), demounted, and then stained with mAb G3 against tryptase (B). As visible, the spindle-shaped tryptase-positive MCs were found to be immunoreactive for CD2. Original magnification approximately × 500. (C,D) Immunocytochemistry of HMC-1 cells. Sorted HMC-1 subsets, ie, CD2+ HMC-1 cells (C) and CD2− HMC-1 cells (D), were spun on cytospin slides and stained with CD2 mAb 6F10.3 as described in the text. Immunocytochemistry confirmed expression of the CD2 protein in the cytoplasm of CD2+ HMC-1 cells and absence of (cytoplasmic) CD2 in the surface CD2− HMC-1 cells. Note granular staining pattern of CD2+ HMC-1 cells. Original magnification approximately × 250.

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