Expression of CD2 epitopes on HMC-1 cells.

HMC-1 cells were incubated with mAbs against different epitopes of CD2. (A) A single-color flow cytometry experiment. As visible, HMC-1 reacted with various CD2 mAbs, including those directed against T11-1 (39C1.5) and T11-2 epitopes (6F10.3). However, HMC-1 cells did not react with mAb VIT13 directed against the T11-3 epitope of CD2. (B) A multicolor staining experiment performed with the PE-labeled mAb RPA-2.10, the FITC-labeled mAb 39C1.5, FITC-labeled mAb 6F10.3, and FITC-labeled mAb VIT13. Multicolor staining confirmed that the CD2 epitopes T11-1 (39C1.5) and T11-2 (6F10.3) are coexpressed on the same (identical) subset of HMC-1.