American Society of Hematology

Fig. 5.

$Fig. 5. Antibody reactivity of sorted HMC-1 fractions. / After sorting for CD2+ and CD2− cells, the HMC-1 subpopulations were kept in culture for 140 days. On day 42, cells were incubated with mAbs directed against the T11-1 epitope (39C1.5), T11-2 epitope (6F10.3), and T11-3 epitope (VIT-13) of CD2 and analyzed by flow cytometry. As visible, the cells retained their phenotype after long-term culture. In fact, the HMC-1 cell subset sorted for CD2 (left panel) remained T11-1+ and T11-2+, whereas the CD2− HMC-1 fraction still was T11-1− and T11-2− on day 42. The same result was obtained after keeping HMC-1 cell subpopulations in culture for 140 days.$

Antibody reactivity of sorted HMC-1 fractions.

After sorting for CD2⁺ and CD2⁻ cells, the HMC-1 subpopulations were kept in culture for 140 days. On day 42, cells were incubated with mAbs directed against the T11-1 epitope (39C1.5), T11-2 epitope (6F10.3), and T11-3 epitope (VIT-13) of CD2 and analyzed by flow cytometry. As visible, the cells retained their phenotype after long-term culture. In fact, the HMC-1 cell subset sorted for CD2 (left panel) remained T11-1⁺ and T11-2⁺, whereas the CD2⁻ HMC-1 fraction still was T11-1⁻ and T11-2⁻ on day 42. The same result was obtained after keeping HMC-1 cell subpopulations in culture for 140 days.

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