Splenic expression of DMT1.

Expression of DMT1 in the spleens of PHZ- (A) or EPO- (B) (48 hour) treated mice and in the spleen of mk/mk (C) mice. Spleen microsomal fractions (80 μg) together with 5 μg membrane proteins prepared from CHO cells or CHO transfectants expressing either a cMyc-tagged DMT1 isoform II (CHO-DMT1) or a cMyc-tagged mNramp1 (CHO-Nramp1) were analyzed by SDS-PAGE and immunoblotting with anti–DMT1-NT (i), anti-TfR (ii), and anti– mNramp1-NT (iii) antisera. The positions and sizes (in kilodaltons) of molecular mass markers are shown.