American Society of Hematology

Fig. 5.

$Fig. 5. DMT1 expression in RBCs of normal mice. / (A-B) Immunoblotting of ghost RBC membranes (80 μg) isolated from control animals (−) or mice treated with EPO (48 hours, +, A) or PHZ (+, B). (C) Western blot analysis of crude membrane fractions (100 μg) obtained from MEL cells. Membrane proteins (5 μg) from control CHO cells (CHO) and CHO transfectant expressing a cMyc-tagged DMT1 isoform II (CHO-DMT1) were used as controls. Immunoblotting was performed with antibodies raised against DMT1-NT (i), DMT1-CT (ii), and TfR (iii). The asterisk indicates the position of an unspecific protein species detected only with our anti–DMT1-CT. The positions and sizes (in kilodaltons) of molecular mass markers are shown.$

DMT1 expression in RBCs of normal mice.

(A-B) Immunoblotting of ghost RBC membranes (80 μg) isolated from control animals (−) or mice treated with EPO (48 hours, +, A) or PHZ (+, B). (C) Western blot analysis of crude membrane fractions (100 μg) obtained from MEL cells. Membrane proteins (5 μg) from control CHO cells (CHO) and CHO transfectant expressing a cMyc-tagged DMT1 isoform II (CHO-DMT1) were used as controls. Immunoblotting was performed with antibodies raised against DMT1-NT (i), DMT1-CT (ii), and TfR (iii). The asterisk indicates the position of an unspecific protein species detected only with our anti–DMT1-CT. The positions and sizes (in kilodaltons) of molecular mass markers are shown.

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