DMT1 expression in red blood cells from microcytic anemia

(mk) mice. Panels A and B represent 2 independent immunoblots of crude membrane fractions from RBCs isolated from heterozygotes mk/+ and from homozygotesmk/mk mice. Membrane proteins from +/+ mice untreated or treated with EPO [+/+(+EPO)] (A), from mk/+mice treated with PHZ [mk/+(+PHZ)] (B), or from CHO cells (CHO) and CHO transfectants expressing DMT1 isoform II (CHO-DMT1) (A and B), were used as controls. Immunoblotting was performed using antibodies raised against DMT1-NT (Ai and Bi) or TfR (Aii and Bii). The positions and sizes (in kd) of molecular mass markers are shown. (Aiii) Percentage of reticulocytes in blood samples isolated from +/+ (1), +/+ (+ EPO) (2), and mk/+ (3) and mk/mk (4). (Biii) Percentage of reticulocytes in blood samples isolated frommk/+(+PHZ) (1), mk/+ (2), andmk/mk (3).