In vivo interaction of AF10 and GAS41.

(A) Immunoblot analysis with a rabbit polyclonal anti-AF10 antibody of the endogenous AF10 and GAS41 immunoprecipitates from whole extract of KG1a cells (lanes 1 and 2, respectively); 30 μg protein extract (3% of that used for immunoprecipitations) was used as antibody positive control (lane 3). Immunoprecipitates with rabbit preimmune serum was used as negative control (lane 4). Samples were run on an 8% polyacrylamide gel. (B) Immunoblot analysis with a rabbit polyclonal anti-GAS41 antibody of AF10 and GAS41 immunoprecipitates from whole extract of KG1a cells (lanes 1 and 2, respectively); 30 μg protein extract was used as antibody positive control (lane 3). Immunoprecipitates with rabbit preimmune serum were used as negative control (lane 4). The closed arrowhead indicates the presence of the light chain (22 KD) of the rabbit antibody. Samples were run on a 15% polyacrylamide gel.