Fig. 2.
Effect of CD30 ligation on NF-κB, SP1, AP-1, and NRF-1 binding activity in L540 cells.
2 × 106 cells/mL were cultured for 30 minutes in 24-well plates coated with 10 μg/mL plastic bound anti-CD30 agonistic mAb M67 or BerH2 as a control and in the absence or presence of PMA–ion (15 ng/mL PMA + 500 ng/mL ionomycin). M67 induced NF-κB but not Sp1, AP-1, or NRF-1 DNA binding activity in nuclear extracts from L540 cells. PMA–ion induced not only NF-κB but also AP-1 binding activity. The BerH2 control was not represented if stimulation with M67 mAb was ineffective. A section of gels is shown. The experiment depicted in this figure is representative of 5.
