The lck transgene cassette contains a

BamHI restriction site behind the lck proximal promoter. The proximal lck promoter directs T cell-specific expression.55 56 BamHI was used to subclone the mCCL21 cDNA into the p1017 plasmid behind the lck proximal promoter.NotI restriction digest was then used to isolate the transgene cassette from the plasmid.