Southern blot of isolated mouse genomic DNA and Western blot of isolated mouse thymocyte cell lysate.

Homozygous transgenic (lck/lck-mCCL21) and heterozygous transgenic (WT/lck-mCCL21) mice were compared to wild-type (WT/WT) mice. Southern analysis of BamHI-digested mouse genomic DNA was performed using radiolabeled mCCL21 cDNA as a probe. Western analysis was performed using antimouse CCL21 IgG-goat as a primary antibody, antigoat IgG-HRP as a secondary antibody, and detected by ECL. On prolonged exposure appropriate endogenous CCL21 expression can be seen on Western analysis, but is not seen on the exposure shown here.