CCL21 and CCL19 chemotaxis of CD3⁺/CD62L⁺ T cells is reduced in homozygous lck/lck-mCCL21 transgenic versus normal mice.

Chemotaxis assays were performed comparing normal (WT/WT) and homozygous transgenic (lck/lck-mCCL21) mouse splenocytes (A-C) and thymocytes (D,E). Dose responses to 0, 200, 400, and 800 ng/mL chemokine were obtained. Flow cytometric analysis was performed to characterize starting and migrating cell populations. Severe deficiencies in transgenic CD3⁺/CD62L⁺splenocyte and thymocyte chemotaxis in response to CCL21 (A,D) and CCL19 (B,E) are observed. Splenocyte chemotactic response to CXCL12 was unchanged (C).