Decreased total nucleated cells in the bone marrow of STAT5A/5B-deficient mice.

Total nucleated cells were obtained from both hind legs, and cell counts were determined after red blood cell lysis. Subsetting was performed according to surface expression of lineage-defining markers and Sca-1. Absolute values were generated by multiplying gated percentages by total nucleated cell numbers. (A) Total nucleated cells (P = .002). (B) Representative FACS analysis to quantitate bone marrow subsets. (C) The lin^dimSca-1⁺compartment, containing HSCs (P = .7). (D) The lin^dimSca-1^neg/lo compartment, containing post-HSC progenitors (P = .002). PE indicates phycoerythrin; FITC, fluorescein isothiocyanate.