Absence of STAT5A/5B^−/− contribution (CD45.2⁺) to granulocytes (Gr-1⁺) in peripheral blood after competitive repopulation.

Peripheral blood from irradiated recipients receiving competitor cells only (A), competitor cells plus STAT5A/5B^+/+ tester cells (B), or competitor cells plus STAT5A/5B^−/− tester cells (C) were analyzed 10 weeks after initiation of competitive repopulation assay. Peripheral blood was stained for CD45.1, CD45.2, and Gr-1 after red blood cell lysis and shown here are representative dot-plots portraying CD45.1 versus CD45.2 expression within the Gr-1⁺ gate.