OPG and OPGL mRNAs are expressed in the BM environment and are regulated by IL-1β.

(A) OPGL, OPG, and β-actin mRNA were analyzed by semiquantitative RT-PCR in BMSCs and in preOBs at 2 to 4 weeks of culture. (B) Effect of IL-1β (10 ng/mL) or IL-6 (100 ng/mL) plus sIL-6Rα (250 ng/mL) on OPGL and OPG mRNA expression by preOBs. The figures are representative of 3 independent RT-PCR reactions. Cont indicates control.