Fig. 4.
The induction of OPGL mRNA expression in coculture involves cell-to-cell contact mediated in part by the integrin VLA-4.
Semiquantitative RT-PCR analysis of OPGL and β-actin mRNA in a coculture system with fixed preOBs or fixed XG-6 (A), in the presence of a transwell insert (B), and in the presence of VLA-4 mAb (10 μg/mL) compared with an IgG control (C). The figures are representative of 3 independent RT-PCR reactions of repeated experiments.