Fig. 4.
Fig. 4. Expression of wild-type and mutant fibrinogens. / Fibrinogens secreted from COS-1 cells, transfected with wild-type Bβ- and γ-chains, together with each mutant (Gly13stop, FS51stop, Ser100stop, Arg110stop, and Arg149stop) Aα-chain were analyzed as shown. Transfections, untransfected HepG2 controls, SDS-PAGE, and Western blot were carried out as described in “Materials and methods.” Proteins were separated on 4% SDS-PAGE under nonreducing conditions. The arrowhead indicates the 340-kd normal fibrinogen.

Expression of wild-type and mutant fibrinogens.

Fibrinogens secreted from COS-1 cells, transfected with wild-type Bβ- and γ-chains, together with each mutant (Gly13stop, FS51stop, Ser100stop, Arg110stop, and Arg149stop) Aα-chain were analyzed as shown. Transfections, untransfected HepG2 controls, SDS-PAGE, and Western blot were carried out as described in “Materials and methods.” Proteins were separated on 4% SDS-PAGE under nonreducing conditions. The arrowhead indicates the 340-kd normal fibrinogen.

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