Effect of iTCC on leukocyte trafficking in rat mesenteric postcapillary venules.
Traffic of leukocytes was monitored at various time intervals in response to the following stimuli: iTCC (1.5 × 10−8 M; ▴), C5a (10−7 M; ░), boiled iTCC (1.5 × 10−8 M; ○), and control saline (⋄). Measurements were performed on 4 to 6 different segments of unbranched venules (25- to 40-μm diameter, 200-μm length). (A) Values of rolling leukocyte flux defined as number of cells that become visible as bright spheres if they travel inside the venules more slowly than red blood cells. (B) Numbers of leukocytes stably adherent to the same site of postcapillary vascular endothelium of rat mesentery for at least 30 seconds. (C) Numbers of cells that migrated to the extravascular space up to 100 μm away from the vessel wall, in parallel with 200-μm vessel segments. *P < .01 versus control.
