Fig. 3.
In vitro transendothelial migration of PMNs induced by iTCC.
Transwell filters coated with first-passage HUVECs were treated for 4 hours at 37°C with increasing amounts of (A) iTCC, boiled iTCC (5 nM), LPS (0.5 μg /mL), or medium added to the lower compartment or (B) iTCC (5 nM), TNF-α (1.5 × 103 U/mL), or medium added to the upper compartment and washed after 4 hours of incubation. PMNs were added to the upper chamber, and the percentage of migrated PMNs was evaluated after an incubation of 30 minutes. Values are means ± SD of triplicate determinations of 3 separate experiments. *P < .01 versus control.
