Scanning electron microscopy of human PMNs adherent to HUVECs.

EC monolayers grown on a glass coverslip were pretreated for 4 hours with either iTCC (5 nM; top) or TNF-α (200 U/mL; bottom), and PMNs were added on top of the monolayers. After 30 minutes of incubation at 37°C, ECs were washed twice with PBS and then fixed with 2.5% glutaraldehyde in 0.2 M cacodylate buffer, pH 7.4.