Rate of intracellular zinc reductions in thymocytes following treatment with iron chelators.

Thymocytes were preloaded with zinquin (25 μM) for 30 minutes before the addition of PBS (control) or iron chelators CP20 or DFO (300 μM IBE). Zinquin fluorescence was measured by spectrofluorimetry at 390 nm, and the results are expressed as a percentage of control. The data shown are the mean ± SD of 4 independent experiments performed in duplicate.