Fig. 3.
Effect of iron chelators on intracellular zinc levels.
Thymocytes were incubated with PBS (control), dexamethazone (10−7 M), or the iron chelators CP20 or DFO (300 μM IBE) for 24 hours. The cells were washed 3 times in PBS prior to the addition of zinquin (25 μM). Zinquin fluorescence was measured by spectrofluorimetry. The data shown are the mean ± SD of 4 independent experiments performed in duplicate.