Fig. 2.
Fig. 2. Real-time RT-PCR quantification for Bcl-2, β-actin, and S9 ribosomal protein mRNAs levels. / Real-time quantitative RT-PCR was performed with appropriate primers for Bcl-2 (A), β-actin (B), and S9 ribosomal protein (C) using SYBR Green I dye. Amplification results were visualized using the Sequence Detector 1.7 software. These are representative plots showing increasing fluorescence (Δ Rn) detected through cycle 40 on normal GC B cell or FL cDNA samples. NTC represents the negative control amplification (using H20 as template). CT represents the threshold cycle at which fluorescence is first detected above background. A higher CT value is indicative of lower mRNA levels (more PCR cycles required to exceed background).

Real-time RT-PCR quantification for Bcl-2, β-actin, and S9 ribosomal protein mRNAs levels.

Real-time quantitative RT-PCR was performed with appropriate primers for Bcl-2 (A), β-actin (B), and S9 ribosomal protein (C) using SYBR Green I dye. Amplification results were visualized using the Sequence Detector 1.7 software. These are representative plots showing increasing fluorescence (Δ Rn) detected through cycle 40 on normal GC B cell or FL cDNA samples. NTC represents the negative control amplification (using H20 as template). CT represents the threshold cycle at which fluorescence is first detected above background. A higher CT value is indicative of lower mRNA levels (more PCR cycles required to exceed background).

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