Fig. 1.
Overexpression of
Dll4. Full-length murine Dll4 cDNA was inserted into the multiple cloning sites in MSCV2.2 (panel A), driven by LTR promoter. Restriction enzymes KpnI andSpeI are illustrated. TM indicates transmembrane region. Total RNA was isolated from 4 control and 5Dll4-overexpressing animals at 6 weeks after bone marrow transplantation (BMT) (panel B). Top panel was probed with32P-labeled Dll4 cDNA, and bottom panel was probed with 32P-labeled β-actin.