Fig. 1.
![Fig. 1. The inhibitory activity of AF, but not CSA, is inversely correlated to stimulus and is overridden by increasing receptor engagement. / Jurkat cells were stimulated with various concentrations of PHA in the absence (▪) or presence of either AF (25% ■) or PP14-depleted AF (▴) (A); or in the presence of CSA (100 pg/mL [■] or 2 ng/mL [▵]) (B). IL-2 secretion was determined in the conditioned medium. The data represent the mean of triplicate samples. Comparable results were obtained in 4 separate experiments. (C) Change in absolute IL-2 levels as a function of PHA concentration in the presence of either AF (25% ▪) or CSA (100 pg/mL [■] or 2 ng/mL [▵]), as calculated from the data in panels A and B.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/13/10.1182_blood.v98.13.3727/6/h82411873001.jpeg?Expires=1735555599&Signature=xPhYkZobSBPzL~nJ6Qpr-JONrA2FjF9Vnzf2sc8TD5cWvZ4bIb2TT3Xg2M5yvuDT55zyBMvxq3rkXUh2kI~hMvQ1jy9bTjk44bpggsj6pqUA03KuCIV7XlnGnOAHXD251aLEr049Ii4Vxl7zBJFhTWfukyPCztSyQ4u7okLfs2fajxT10D~WNY6vy10hCdDy9fiI4wPnbksFnktU-GAaE-H0kFVD93mvbFW4xr9iyGU75ILwEwrDx7qctNR1GKxKXk5Y9q8MQtC~yi9rzyYaTsjrtjHUkw3AOTj9xPefNGPyzGmg-zew2V6fBT8Ig73QPlnXsZ-Q-daV8calYxNmQQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The inhibitory activity of AF, but not CSA, is inversely correlated to stimulus and is overridden by increasing receptor engagement.
Jurkat cells were stimulated with various concentrations of PHA in the absence (▪) or presence of either AF (25% ■) or PP14-depleted AF (▴) (A); or in the presence of CSA (100 pg/mL [■] or 2 ng/mL [▵]) (B). IL-2 secretion was determined in the conditioned medium. The data represent the mean of triplicate samples. Comparable results were obtained in 4 separate experiments. (C) Change in absolute IL-2 levels as a function of PHA concentration in the presence of either AF (25% ▪) or CSA (100 pg/mL [■] or 2 ng/mL [▵]), as calculated from the data in panels A and B.
