The role of PI 3–kinase in regulating platelet spreading under static conditions.
Washed platelets (1.5 × 108/mL for panels A and B or 1.5 × 107/mL for panel C) were incubated with vehicle alone (control) or the indicated concentrations of LY294002 (0-25 μM) or wortmannin (0-100 nM) for 15 minutes. These results demonstrate (A) the mean surface area of adherent platelets, and (B) the morphology of spread platelets as determined by scanning electron microscopy (bar = 2 μm). Statistical analysis of the results was performed using a t test and the P values are indicated where appropriate (P < .05*). Results are the mean ± SEM of 3 experiments, and images are from a single experiment representative of 3. In panel C, platelet spreading was visualized in real time by differential interference contrast microscopy and recorded on video for off-line analysis (× 100 oil objective; bar = 5 μm). The images presented are typical of cells from a single experiment and are representative of 3 independent experiments.
