Fig. 7.
Fig. 7. The role of PI 3–kinase in regulating platelet thrombus formation under flow conditions. / Anticoagulated whole blood was incubated with vehicle alone (control) or the indicated concentrations of LY294002 (0-200 μM) for 15 minutes prior to perfusion over immobilized VWF (100 μg/mL) at 1800 s-1. In panel A, thrombi were fixed and incubated with DiOC6. Platelet thrombi were reconstructed using a computer-assisted image analysis program. The upper panels represent an oblique view to demonstrate surface coverage; the lower panels represent a side-on view to demonstrate differences in thrombus height. The results in panel B demonstrate the effect of PI 3–kinase inhibition on thrombus formation.

The role of PI 3–kinase in regulating platelet thrombus formation under flow conditions.

Anticoagulated whole blood was incubated with vehicle alone (control) or the indicated concentrations of LY294002 (0-200 μM) for 15 minutes prior to perfusion over immobilized VWF (100 μg/mL) at 1800 s-1. In panel A, thrombi were fixed and incubated with DiOC6. Platelet thrombi were reconstructed using a computer-assisted image analysis program. The upper panels represent an oblique view to demonstrate surface coverage; the lower panels represent a side-on view to demonstrate differences in thrombus height. The results in panel B demonstrate the effect of PI 3–kinase inhibition on thrombus formation.

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