Fig. 8.
Tax expression is down-regulated but p24 is up-regulated after arsenic trioxide treatment.
MT-2 cells were treated with control buffer, IFN-α, As2O3, or As2O3 + IFN-α for 48 hours. Sixty micrograms total protein was then resolved on 16% Tris-glycine gels transferred to PVDF membranes and probed with specific antibodies for (A) Tax or (B) Gag p24. Comparable protein loading was verified using an antibody specific for the β-tubulin protein (C).
