Up-regulation of p21 mRNA during the terminal differentiation of CFU-M.

Individual human adult bone marrow cells were sorted by flow cytometry and cultured under the stated conditions. Five single cells isolated by micromanipulation and harvested at each time point from the emerging CFU-M colonies were analyzed using RT-PCR and Southern blotting. Quantitative analysis of p21 normalized to GAPDH signals presented in the graph was performed using PhosphorImager-generated quantitation of signal intensity when the poly-dT–primed RT-PCR product was probed with radiolabeled p21 or GAPDH. Data shown are from 1 of 3 similar experiments with similar results.