Studies of RANKL expression by 5T2MM cells.

(A) RT-PCR analysis of RNA isolated from purified 5T2MM cells (5T2) or bone marrow mononuclear cells from the vertebrae (BM1) or femora (BM2) of control, uninjected mice. M represents a lane containing a DNA standard, and Con is a negative control. (B) Flow cytometric analysis of idiotype-positive 5T2MM cells isolated from the bone marrow of mice bearing 5T2MM cells. Black histogram shows RANKL staining, and white histogram shows staining with an isotype-matched control antibody.