Transduction of the human T-lymphoid cell line Molt-3 with retroviral vectors carrying the CD2 LCR and unmodified LTRs does not change the EGFP expression pattern.

Molt-3 cells were infected with LESN or the LCR-containing retroviral vectors generated by transient transfection of 293T cells and were selected in G418-containing medium for 4 weeks before FACS analysis. LESN-LCR and LESN-LCRas vectors carry the LCR element in the sense or antisense orientation, respectively. Representative patterns of EGFP expression are shown. The percentage of EGFP-positive cells for each construct and MFI are indicated, as is the CV, which describes the profile of the bell-shaped curve. The pattern of the mock-transduced lymphoid cells in each panel is also shown.