Quantification of gene transfer rates and analysis of proviral DNA structure by Southern blot analysis of transduced Molt-3 cells.

DNA was isolated from bulk cultures of vector-transduced Molt-3 cells in the presence of G418 selection, digested with KpnI that cuts once in each LTR, and analyzed by Southern blotting. The blot was first hybridized with a probe for neoR (upper panel), stripped, and rehybridized with a loading control probe, human CXCR4 (lower panel). Band intensities were quantified with an InstantImager (Packard) and were used to calculate the relative number of provirus copies per genome in each sample, which is indicated. DNA from untransduced Molt-3 cells was used as control (mock).