Fig. 5.
Effect of LIBS6-induced platelet aggregation on thromboxane A2 generation.
Human platelets were treated with Fab fragments of LIBS6 Fab (600 nM) at 37°C with stirring for 5 minutes. Fibrinogen (3 μM) was then added, and stirring was continued for an additional 3 minutes. The reaction was stopped by snap-freezing, and thromboxane B2was measured as described in Figure 1. The results were from 3 independent experiments in duplicate.
