EMSA using LILRE as radiolabeled probe.

(A) EMSA of nuclear extracts isolated from unstimulated (lanes 1 and 4), IL-6–stimulated (lane 2), and IFN-γ–stimulated (lane 3) THP-1 cells. The sample in lane 4 has been incubated with STAT1N (G16930) antibodies. (B) EMSA of nuclear extracts isolated from IL-6–stimulated (lanes 1-4) B1 cells. To assess specificity of LILRE oligobinding, a 100-fold molar excess of unlabeled LILRE, hSIE, and FcγRI oligonucleotides (lanes 1, 2, and 3) was added to compete for radiolabeled LILRE binding. Furthermore, NF-κB (p52) antibodies were included as control for nonspecific antibody binding (lane 4). Figures represent the results of 3 independent experiments (n = 3). MW indicates molecular weight.